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Associate Professor Bostjan Kobe
School of Chemistry and Molecular Biosciences, and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology)
University of Queensland
Brisbane, Qld 4072
Email: b.kobe@uq.edu.au
Tel: +61-7-3365-2132
Fax: +61-7-3365-4699
Homepage: http://www.smms.uq.edu.au/bmb
Research |
We are interested in structure, function and regulation of proteins and macromolecular assemblies, focusing on structural approaches and the use of structural information. Research on proteases constitutes a small part of our research. For example, we have determined the crystal structure of latexin, a carboxypeptidase inhibitor, and characterized its binding to caroboxypeptidase using a hybrid approach employing chemical crosslinking, mass spectrometry and molecular modeling. We also analyzed the interaction of the thrombin with the leucine-rich repeat domain of glycoprotein Ibalpha.
Many proteases are autoregulated via autoinhibition through pre-pro-sequences. This type of control falls into the broader mechanism termed intrasteric or active site-directed regulation. We have characterized the related regulatory mechanisms in a number of systems including protein kinases, metabolic enzymes and transport factors. |
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Collaborations |
Professor Jenny Martin, University of Queensland, Australia
Professor David Fairlie, University of Queensland, Australia |
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Publications |
Kobe, B. and Kemp, B.E. (1999). Active-site directed protein regulation. Nature 402, 373-376
Aagaard A, Listwan P, Cowieson N, Huber T, Ravasi T, Wells C, Flanagan JU, Kellie S, Hume DA, Kobe B and Martin JL (2005) An inflammatory role for the mammalian carboxypeptidase inhibitor latexin: relationship to cystatins and the tumour suppressor TIG1.
Structure 13: 309-317
Mouradov D, Craven A, Forwood JK, Flanagan JU, Garcia-Castellanos R, Gomis-Ruth FX, Hume DA, Martin JL, Kobe B and Huber T (2006) Modelling the structure of latexin-carboxypeptidase A complex based on chemical cross-linking and molecular docking. Protein Eng Des Sel 19: 9-16
Kobe B, Guncar G, Buchholz R, Huber T and Maco B (2009) The many faces of platelet glycoprotein Ibalpha - thrombin interaction. Curr Prot Pept Sci, in press |
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