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Methods

Plant Tissue Culture (Anderson, La Trobe Uni)

Ubiquitin reagents (Baker, ANU)

Yeast genetics/molecular biology (Baker, ANU)

Yeast Systems: for analysing caspase activity (Hawkins, MCRI)

Tools

Glasshouse Facilities (Anderson, La Trobe Uni)

Confocal Microscopy (Baker, ANU)

X-ray Diffractometer (Blanchard, Griffith Uni)

Beckman Coulter Proteomics Lab (Clements, QUT)

500 and 600 MHz spectrometer (Craik, UQ)

ACRF proteomic facility:under development (Kumar, IMVS)

Biophotonic Imager (Lavin, QIMR)

Crystallisation and X-ray Facilities: intense lab-based X-ray source, plus chromium anode for SAD structure determination. (Martin, UQ)

BMG Fluorostar and Polarstar fluorescent plate readers: limited time available (Pike, Monash)

Arcturus (Stage I) PixCell II system for laser capture microdissection (Thompson, UniMelb)

High resolution (20u focal spot) Faxitron for skeletal monitoring (Thompson, UniMelb)

Collaborative access to nearby (REEV) ultra-high resolution (5 u spot), Phase Contrast Imaging (PI) X-Ray technology (Thompson, UniMelb)

Fuji BAS 5000 (25 micron resolution) for analysis of 33p-radiolabelled gene filters (Thompson, UniMelb)

Perkin Elmer SDS 5700 "TaqMan" system for Real Time Quantitative RT-PCT (RTQ-RT-PCR) (Thompson, UniMelb)

Axon Fluorescent Scanner (Thompson, UniMelb)

HPLC (Smith, Baker Inst.)

MALDI-TOF-MS (Smith, Baker Inst.)

Electrospray-MS (Smith, Baker Inst.)

Cell sorter, FACS, HPLC, FPLC (Stewart, UWA)

FACS sorting facilities (Villadangos, WEHI)

Genetic analysis facilities (AGTC) (Villadangos, WEHI)

Fuji FLA5000 Laser Fluorescence scanner/Phosphorimager (Watters, Griffith Uni)

Perkin Elmer Viktor 2 Multiplate reader: luminescence/fluorescence/absorbance (Watters, Griffith Uni)

Nikon Eclipse E800 fluorescence microscope with Normarski Fuji LAS 3000 Chemiluminescence detector (Watters, Griffith Uni)

Technologies

Insect Rearing (Anderson, La Trobe Uni)

Cloning and expression facilities: bacterial, baculovirus and plants (Anderson, La Trobe Uni)

Recombinant Protein Expression: bacteria,yeast (Baker)

Protein Crystallography (Blanchard, Griffith Uni)

High throughput protein expression screening (Martin, UQ)

High Throughput Proteomics (Smith, Baker Inst.)

Peptide Biochemistry (Smith, Baker Inst.)

Physiology Protein Engineering (Smith, Baker Inst.)

Enzyme assay development (Smith, Baker Inst.)

Cell Culture (Smith, Baker Inst.)

Cell Biology (Smith, Baker Inst.)

Cloning and Expression (Smith, Baker Inst.)

Edman sequencing (Smith, Baker Inst.)

Confocal microscopy (Stewart, UWA)

Proteomics (Villadangos, WEHI)

Mouse genetic manipulation (transgenic, knock-outs) (Villadangos, WEHI)

Antibody production (Villadangos, WEHI)

Recombinant protein production (Villadangos, WEHI)

Cell Lines

ADAMTS-1 knockout mice (Pritchard, Monash)

Mouse Model for Menstruation (Salamonsen, Monash)

Tissue Banks

The Queensland Brain Bank has been operating since 1989. More recently it has included a node of the NHMRC Neural Tissue Repository (NTR) and the Queensland branch of Use Your Brains (UYB). The original bank was primarily designed to collect frozen tissue from selected brain regions from controls; alcoholic cases and co-morbidities; and Alzheimer disease and related dementia cases. The brief of the NTR is broader in terms of diseases, but focuses on the dementias plus rarer diseases that can be expected infrequently in any jurisdiction: currently there are four nodes, in Melbourne, Sydney, Adelaide and Brisbane. UYB is an initiative originating in Sydney which aims to accumulate brain tissue from the well elderly; it is currently only at a very early stage. Our overall aims include having a database integrated with the National NTR system. Enquiries should be directed to Sonja Webb RN, , 07-3365-4614.

Gene Banks

Alfred and Baker Gene Bank has been operating since January 2000. It's key objective is to facilitate research into the genetics of cardiovascular disease by developing a bank of well-characterised samples with good clinical correlates. The Gene and tissue Bank relies on blood and tissue donations from healthy volunteer subjects in addition to people who have already had a heart attack, stroke or have high blood pressure, high cholesterol or other risk factors for cardiovascular disease. To date over 3,500 samples have been registered. More information can be obtained on 61 3 9276 3895.

Preclinical Testing

Baker Heart Research Institute
Preclinical testing for cardiovascular disease (More Information).





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